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Image Search Results
Journal: Frontiers in Immunology
Article Title: Integrin activating molecule-talin1 promotes skin fibrosis in systemic sclerosis
doi: 10.3389/fimmu.2024.1400819
Figure Lengend Snippet: Transcriptome analysis reveals enhanced talin1 expression among integrin activating molecules in the skin tissues of patients with SSc. (A) mRNA expression levels of talin1 between SSc and HCs. (B) mRNA expression levels of talin2 between SSc and HCs. (C) mRNA expression levels of kindlin1 between SSc and HCs. (D) mRNA expression levels of kindlin2 between SSc and HCs. (E) mRNA expression levels of kindlin3 between SSc and HCs. (F) Correlation between expression of integrin activating molecules with clinical characteristics in GEO database. * indicates P<0.05; ** indicates P<0.01; *** indicates P<0.001; **** indicates P<0.0001; ns indicates P>0.05.
Article Snippet: Serum talin1 levels were measured with a specific
Techniques: Expressing
Journal: Frontiers in Immunology
Article Title: Integrin activating molecule-talin1 promotes skin fibrosis in systemic sclerosis
doi: 10.3389/fimmu.2024.1400819
Figure Lengend Snippet: Talin1 is highly expressed in skin fibroblasts in SSc. (A) HE, Masson trichrome, and immunofluorescence staining of skin tissues between SSc and HCs. (B) The MFI of talin1 between SSc and HCs. (C) The percentage of talin1 positive cells between SSc and HCs. (D) MFI of talin1 was positively correlated with mRSS in SSc. (E) Double immunofluorescence staining talin1 and vimentin in SSc and HCs. (F) The percentage of talin1-positive fibroblasts in SSc and HCs. (G) Relative protein levels of talin1 in the skin fibroblast between SSc and HCs. (H) Semi-quantification of the western blot results by ImageJ software.
Article Snippet: Serum talin1 levels were measured with a specific
Techniques: Immunofluorescence, Staining, Double Immunofluorescence Staining, Western Blot, Software
Journal: Frontiers in Immunology
Article Title: Integrin activating molecule-talin1 promotes skin fibrosis in systemic sclerosis
doi: 10.3389/fimmu.2024.1400819
Figure Lengend Snippet: Correlation of serum soluble talin1 levels with clinical features and symptoms in SSc patients.
Article Snippet: Serum talin1 levels were measured with a specific
Techniques:
Journal: Frontiers in Immunology
Article Title: Integrin activating molecule-talin1 promotes skin fibrosis in systemic sclerosis
doi: 10.3389/fimmu.2024.1400819
Figure Lengend Snippet: Talin1 promotes the activation and migration of skin fibroblasts. (A, B) Expression and quantification of talin1 in α-SMA positive cells in the skin of SSc and HCs. (C) Relative transcript levels of talin1, α-SMA and COL1A1 measured by real-time PCR in SSc patients’ fibroblasts with or without talin1 knockdown. (D) Relative protein levels of talin1, α-SMA and COL1A1 in SSc patients’ fibroblasts with or without talin1 knockdown. (E) Semi-quantification of the western blot results by ImageJ. (F) Scratch assay was performed on primary fibroblast treated with or without talin1 siRNA. (G) Quantification of migrated SSc patients’ fibroblasts with or without talin1 knockdown. (H) Detection of apoptosis and necrosis using Annexin V-APC and 7AAD dual staining on SSc patients’ fibroblasts with or without talin1 knockdown. (I) Bar graph for apoptotic cell percentages. (J) CCK-8 cell viability assay of SSc patients’ fibroblasts with or without talin1 knockdown.
Article Snippet: Serum talin1 levels were measured with a specific
Techniques: Activation Assay, Migration, Expressing, Real-time Polymerase Chain Reaction, Knockdown, Western Blot, Wound Healing Assay, Staining, CCK-8 Assay, Viability Assay
Journal: Frontiers in Immunology
Article Title: Integrin activating molecule-talin1 promotes skin fibrosis in systemic sclerosis
doi: 10.3389/fimmu.2024.1400819
Figure Lengend Snippet: Talin1 activated the integrin family signaling pathway in SSc. (A) Correlation between expression of talin1 with integrin β families molecules in GEO database. (B) Relative transcript levels of ITGB1, ITGB2 and ITGB5 measured by real-time PCR in knock-down fibroblasts. (C) Expression of integrin β1 and integrin β5 in talin1 positive cells. (D) Quantification of integrin β1 in talin1 positive cells in the skin of SSc and HCs. (E) Quantification of integrin β5 in talin1 positive cells in the skin of SSc and HCs. ** indicates P<0.01; *** indicates P<0.001; **** indicates P<0.0001.
Article Snippet: Serum talin1 levels were measured with a specific
Techniques: Expressing, Real-time Polymerase Chain Reaction, Knockdown